Metabolomics Research Laboratory

Research University

Under the program “Excellence Initiative – Research University” a new core facility – Metabolomics Research Laboratory – is being established at the Medical University of Gdańsk. The unit was established through the experience of employees of the Department of Biopharmacy and Pharmacodynamics and the Department of Biopharmacy and Pharmacokinetics, which were set up within the structure of the Faculty of Pharmacy of the Medical University of Gdańsk. In addition to advanced biomedical analyses and pharmacometabolomics research, the Laboratory offers consultations for research and development projects to faculty members, Ph.D. students, and external institutions.

The research team consists of experienced scientists:

Dr. Habil. Danuta Siluk, Associate Professor
Wiktoria Struck-Lewicka, Ph.D.
Dr. Habil. Danuta Dudzik
Renata Wawrzyniak, Ph.D.
Małgorzata Waszczuk-Jankowska, Ph.D.
Kinga Westphal, Ph.D.
Joanna Raczak-Gutknecht, Ph.D.
Paweł Wityk, Ph.D.
Szymon Macioszek, M.A.
Małgorzata Artymowicz, M.A.
Joanna Dawidowska, M.A.

 

Our potential

1. Gas chromatograph coupled with a GCMS mass spectrometer, model TQ 8030 (SHIMADZU)
2. 1260 Infinity liquid chromatography, coupled to a mass spectrometer with a triple quadrupole analyzer, model 6430 Triple Quad LC / MS, Agilent Technologies
3. Liquid chromatograph 1290 Infinity II, coupled with a mass spectrometer with a QTOF analyzer, model 6546 LC-QTOF / MS, Agilent Technologies

Range of services

By combining two advanced research techniques, gas chromatography-mass spectrometry (GC-MS), including electron impact ionization with the use of a quadrupole mass analyzer, and liquid chromatography-mass spectrometry (LC-MS), and (LC-QTOF-MS), the laboratory enables advanced biomedical analyses, in particular:

  • non-targeted qualitative analyses using the fingerprinting/footprinting technique in diverse biological material: biological fluids e.g. blood plasma, urine; tissues e.g. cancer tumors; cell cultures, etc. which enables comprehensive examination and comparison of metabolic profiles of ill and healthy persons. On the one hand, the analyses enable cognitive research on the pathogenesis, but on the other hand, they are also an ideal tool used for searching for potential biomarkers and predictive factors of particular conditions, e.g. civilization diseases (diabetes, cardiovascular diseases, cancer, etc.) as well as any other disease entity.
  • pharmacometabolomics studies
  • analyses consisting in tracking individual metabolic pathways, so-called metabolic profiling
  • analyses consisting in determination of xenobiotic degradation products
  • analyses consisting in searching for new products of biodformation of compounds
  • analyses consisting in the identification of impurities and derivatives of newly synthesized compounds
  • targeted analyses of selected compounds, e.g. analyses of fatty acids
  • development of new methods based on LC-MS and GC-MS techniques
  • processing of LC-MS and GC-MS data and identification of chemical compounds.

 

PRICE LIST

The laboratory will perform analyses according to individual orders with adaptation to the specific needs of contractors. The cost of the service is priced individually.

 

Publications based on research results obtained using our research equipment

1. The potential role of fatty acids in prostate cancer determined by GC-MS analysis of formalin-fixed paraffin-embedded tissue samples 
MAGDALENA BUSZEWSKA-FORAJTA, JOANNA RACZAK-GUTKNECHT, MAŁGORZATA ARTYMOWICZ, WOJCIECH WESOŁOWSKI, KAMIL BUCZKOWSKI, EWA IŻYCKA-ŚWIESZEWSKA, MICHAŁ J MARKUSZEWSKI – J Pharm Biomed Anal 2021 Jan 19;196:113907.

2. GC-MS-based untargeted metabolomics of plasma and urine to evaluate metabolic changes in prostate cancer 
WIKTORIA STRUCK-LEWICKA, RENATA WAWRZYNIAK, MAŁGORZATA ARTYMOWICZ, MARTA KORDALEWSKA, MARCIN MARKUSZEWSKI, MARCIN MATUSZEWSKI, PIOTR GUTKNECHT, JANUSZ SIEBERT, MICHAŁ JAN MARKUSZEWSKI. – J. Breath Res. 2020; vol. 14, nr 4, art. 047103, s. 1-13

3. Paraffin-embedded tissue as a novel matrix in metabolomics study: optimization of metabolite extraction method 
MAGDALENA BUSZEWSKA-FORAJTA, MAŁGORZATA PATEJKO, SZYMON MACIOSZEK, DAWID SIGORSKI, EWA IŻYCKA-ŚWIESZEWSKA, MICHAŁ J. MARKUSZEWSKI. – Chromatographia 2019; vol. 82, nr 10, s. 1501-1513.

4. Metabolomic Heterogeneity of Urogenital Tract Cancers Analyzed by Complementary Chromatographic Techniques Coupled with Mass Spectrometry 
ARLETTE YUMBA-MPANGA, WIKTORIA STRUCK-LEWICKA, RENATA WAWRZYNIAK, MARCIN MARKUSZEWSKI , MAREK ROSLAN, ROMAN KALISZAN , MICHAŁ JAN MARKUSZEWSKI Curr Med Chem 2019;26(1):216-231.

5. Targeted metabolomics in bladder cancer: from analytical methods development and validation towards application to clinical samples 
Yumba Mpanga Arlette, SILUK DANUTA, JACYNA JULIA, Szerkus Oliwia, WAWRZYNIAK RENATA, MARKUSZEWSKI MARCIN, MATUSZEWSKI MARCIN, KALISZAN ROMAN, MARKUSZEWSKI MICHAŁ JAN., Anal. Chim. Acta 2018 : vol. 1037, s. 188-199, 33.

6. Urine metabolic fingerprinting using LC-MS and GC-MS reveals metabolite changes in prostate cancer: A pilot study 
WIKTORIA STRUCK-LEWICKA, MARTA KORDALEWSKA, RENATA BUJAK, ARLETTE YUMBA MPANGA, MARCIN MARKUSZEWSKI, JULIA JACYNA, MARCIN MATUSZEWSKI, ROMAN KALISZAN, MICHAŁ J MARKUSZEWSKI – J Pharm Biomed Anal 2015;111:351-61.

 

CONTACT

Małgorzata Waszczuk-Jankowska, Ph.D.
Division of Pharmacodynamics
Medical University of Gdańsk
Phone: 58 349 1493
malgorzata.waszczuk-jankowska@gumed.edu.pl